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LIPE Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, FC, E |
|---|---|
| Primary Accession | Q05469 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 116598 Da |
| Antigen Region | 822-849 aa |
| Gene ID | 3991 |
|---|---|
| Other Names | Hormone-sensitive lipase, HSL, LIPE |
| Target/Specificity | This LIPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 822-849 amino acids from the C-terminal region of human LIPE. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | LIPE Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | LIPE |
|---|---|
| Function | Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters (PubMed:15716583, PubMed:15955102, PubMed:19800417, PubMed:8812477). Shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs (PubMed:19800417). Preferentially hydrolyzes FA esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs (By similarity). Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor (By similarity). In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production (By similarity). |
| Cellular Location | Cell membrane. Membrane, caveola. Cytoplasm, cytosol. Lipid droplet {ECO:0000250|UniProtKB:P54310}. Note=Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Phosphorylation by AMPK reduces its translocation towards the lipid droplets (By similarity) {ECO:0000250|UniProtKB:P54310, ECO:0000269|PubMed:17026959} |
| Tissue Location | Testis.. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
LIPE has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.
References
Bezaire, V., et al. FEBS Lett. 583(18):3045-3049(2009)
Chen, H.H., et al. Am. J. Clin. Nutr. 90(2):255-262(2009)
Kuzmin, A., et al. Biol. Reprod. 81(2):319-326(2009)
Bezaire, V., et al. J. Biol. Chem. 284(27):18282-18291(2009)
Drenos, F., et al. Hum. Mol. Genet. 18(12):2305-2316(2009)
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