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COG2 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - COG2 Antibody (N-term) AP9379a
    Anti-COG2 Antibody (N-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - COG2 Antibody (N-term) AP9379a
    COG2 Antibody (N-term) (RB17913) IHC analysis in formalin fixed and paraffin embedded human Lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the COG2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
  • FC - COG2 Antibody (N-term) AP9379a
    COG2 Antibody (N-term) (Cat. #AP9379a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q14746
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 83208 Da
Antigen Region 96-124 aa
Additional Information
Gene ID 22796
Other Names Conserved oligomeric Golgi complex subunit 2, COG complex subunit 2, Component of oligomeric Golgi complex 2, Low density lipoprotein receptor defect C-complementing protein, COG2, LDLC
Target/Specificity This COG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 96-124 amino acids from the N-terminal region of human COG2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCOG2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name COG2
Synonyms LDLC
Function Required for normal Golgi morphology and function.
Cellular Location Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side
Research Areas
Citations (0)
citation

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Background

COG2 encodes a subunit of the conserved oligomeric Golgi complex that is required for maintaining normal structure and activity of the Golgi complex. The encoded protein specifically interacts with the USO1 vesicle docking protein and may be necessary for normal Golgi ribbon formation and trafficking of Golgi enzymes.

References

Edwards, T.L., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (5), 721-735 (2009)
Luke, M.M., et al. Stroke 40(2):363-368(2009)
Shiffman, D., et al. Arterioscler. Thromb. Vasc. Biol. 28(1):173-179(2008)
Sohda, M., et al. Traffic 8(3):270-284(2007)
Ungar, D., et al. Trends Cell Biol. 16(2):113-120(2006)

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$ 365.00
$ 140.00
Cat# AP9379a
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