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CROT Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, FC, E |
|---|---|
| Primary Accession | Q9UKG9 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 70178 Da |
| Antigen Region | 49-79 aa |
| Gene ID | 54677 |
|---|---|
| Other Names | Peroxisomal carnitine O-octanoyltransferase, COT, CROT, COT |
| Target/Specificity | This CROT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the N-terminal region of human CROT. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CROT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CROT |
|---|---|
| Synonyms | COT |
| Function | Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester. |
| Cellular Location | Peroxisome. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CROT encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.
References
Li, Y.L., et al. FEBS J. 276(1):303-314(2009)
Morillas, M., et al. J. Biol. Chem. 277(13):11473-11480(2002)
van der Leij, F.R., et al. Mol. Genet. Metab. 71 (1-2), 139-153 (2000) :
Ferdinandusse, S., et al. Biochem. Biophys. Res. Commun. 263(1):213-218(1999)
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