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Phospho-RPS6KA1 (Ser380) Recombinant Rabbit mAb

Phospho-RPS6KA1 (Ser380) Recombinant Rabbit mAb

     
  • WB - Phospho-RPS6KA1 (Ser380) Recombinant Rabbit mAb AP94692
    Sample: Lane 1: A431 cell lysates Primary: Anti-Phospho-RPS6KA1 (Ser380) (AP94692) at 1/1000 dilution Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution Predicted band size: 81 kD Observed band size: 81 kD
    detail
  •  - Phospho-RPS6KA1 (Ser380) Recombinant Rabbit mAb AP94692
    A431 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-RSK1(S380)) monoclonal Antibody, Unconjugated (AP94692) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
    detail
  •  - Phospho-RPS6KA1 (Ser380) Recombinant Rabbit mAb AP94692
    293T cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-RSK1(S380)) monoclonal Antibody, Unconjugated (AP94692) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
    detail
  •  - Phospho-RPS6KA1 (Ser380) Recombinant Rabbit mAb AP94692
    Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Phospho-RSK1(S380)) monoclonal Antibody, Unconjugated (AP94692) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
    detail
  • IHC-P - Phospho-RPS6KA1 (Ser380) Recombinant Rabbit mAb AP94692
    Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-RPS6KA1 (Ser380)) Monoclonal Antibody, Unconjugated (AP94692) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
  • IHC-P - Phospho-RPS6KA1 (Ser380) Recombinant Rabbit mAb AP94692
    Paraformaldehyde-fixed, paraffin embedded (human colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-RPS6KA1 (Ser380)) Monoclonal Antibody, Unconjugated (AP94692) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC
Reactivity Human
Host Rabbit
Clonality Recombinant
Additional Information
Dilution WB=1:1000-5000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:50,IF=1:100-500
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
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Background

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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$ 385.00
Cat# AP94692
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