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SGSH Antibody (C-Term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SGSH Antibody (C-Term) AP9579b
    Anti-SGSH Antibody (C-Term) at 1:4000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P51688
Other Accession NP_000190
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 56695 Da
Antigen Region 420-449 aa
Additional Information
Gene ID 6448
Other Names N-sulphoglucosamine sulphohydrolase, Sulfoglucosamine sulfamidase, Sulphamidase, SGSH, HSS
Target/Specificity This SGSH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 420-449 amino acids from the C-terminal region of human SGSH.
Dilution WB~~1:4000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSGSH Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SGSH
Synonyms HSS
Function Catalyzes a step in lysosomal heparan sulfate degradation.
Cellular Location Lysosome.
Research Areas
Citations (0)
citation

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Background

SGSH is one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with Sanfilippo syndrome A, one type of the lysosomal storage disease mucopolysaccaridosis III, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined.

References

?Sleat, D.E., et al. Mol. Cell Proteomics 5(4):686-701(2006)
?Bekri, S., et al. J. Inherit. Metab. Dis. 28(4):601-602(2005)
?Muschol, N., et al. Hum. Mutat. 23(6):559-566(2004)
?Zhang, H., et al. Nat. Biotechnol. 21(6):660-666(2003)

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$ 365.00
$ 140.00
Cat# AP9579b
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