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ERF3B Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q8IYD1 |
| Other Accession | Q8R050, P15170 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 68883 Da |
| Antigen Region | 216-245 aa |
| Gene ID | 23708 |
|---|---|
| Other Names | Eukaryotic peptide chain release factor GTP-binding subunit ERF3B, Eukaryotic peptide chain release factor subunit 3b, eRF3b, G1 to S phase transition protein 2 homolog, GSPT2, ERF3B |
| Target/Specificity | This ERF3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-245 amino acids from the Central region of human ERF3B. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ERF3B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GSPT2 |
|---|---|
| Synonyms | ERF3B |
| Function | GTPase component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons UAA, UAG and UGA (PubMed:15987998, PubMed:11524954, PubMed:17562865). GSPT2/ERF3B mediates ETF1/ERF1 delivery to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed:15987998). GTP hydrolysis by GSPT2/ERF3B induces a conformational change that leads to its dissociation, permitting ETF1/ERF1 to accommodate fully in the A-site (PubMed:15987998). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:19417104). |
| Cellular Location | Cytoplasm. |
| Tissue Location | Highly expressed in IUCC stage II colorectal cancer (CRC). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
ERF3B encodes a GTPase that belongs to the GTP-binding elongation factor family. The encoded protein is a polypeptide release factor that complexes with eukaryotic peptide chain release factor 1 to mediate translation termination. This protein may also be involved in mRNA stability.
References
Cheng, Z., et al. Genes Dev. 23(9):1106-1118(2009)
Zhouravleva, G., et al. IUBMB Life 58(4):199-202(2006)
Chauvin, C., et al. Mol. Cell. Biol. 25(14):5801-5811(2005)
Hoshino, S., et al. J. Biol. Chem. 274(24):16677-16680(1999)
Hansen, L.L., et al. Cytogenet. Cell Genet. 86 (3-4), 250-251 (1999)
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