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ZRANB1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | Q9UGI0 |
| Other Accession | Q7M760, A6QP16 |
| Reactivity | Human, Mouse |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 80967 Da |
| Antigen Region | 665-692 aa |
| Gene ID | 54764 |
|---|---|
| Other Names | Ubiquitin thioesterase ZRANB1, TRAF-binding domain-containing protein, hTrabid, Zinc finger Ran-binding domain-containing protein 1, ZRANB1, TRABID |
| Target/Specificity | This ZRANB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 665-692 amino acids from the C-terminal region of human ZRANB1. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ZRANB1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ZRANB1 {ECO:0000303|PubMed:21834987, ECO:0000312|HGNC:HGNC:18224} |
|---|---|
| Function | Ubiquitin thioesterase, which specifically hydrolyzes 'Lys- 29'-linked and 'Lys-33'-linked diubiquitin (PubMed:22157957, PubMed:23827681, PubMed:25752573, PubMed:25752577). Also cleaves 'Lys- 63'-linked chains, but with 40-fold less efficiency compared to 'Lys- 29'-linked ones (PubMed:18281465). Positive regulator of the Wnt signaling pathway that deubiquitinates APC protein, a negative regulator of Wnt-mediated transcription (PubMed:18281465). Acts as a regulator of autophagy by mediating deubiquitination of PIK3C3/VPS34, thereby promoting autophagosome maturation (PubMed:33637724). Plays a role in the regulation of cell morphology and cytoskeletal organization (PubMed:21834987). Required in the stress fiber dynamics and cell migration (PubMed:21834987). |
| Cellular Location | Cytoplasm. Nucleus. Note=Enriched in punctate localization in the cytoplasm. |
| Tissue Location | Widely expressed.. |
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Provided below are standard protocols that you may find useful for product applications.
Background
ZRANB1 is a positive regulator of the Wnt signaling pathway that specifically cleaves 'Lys-63'-linked ubiquitin chains. It acts by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription and may also modulate TNF-alpha signaling.
References
Tran, H., et al. Genes Dev. 22(4):528-542(2008)
Evans, P.C., et al. Biochem. J. 357 (PT 3), 617-623 (2001)
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