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DCP1B Antibody (center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DCP1B Antibody (center) AP9888c
    All lanes : Anti-DCP1B Antibody (center) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • FC - DCP1B Antibody (center) AP9888c
    DCP1B Antibody (center) (Cat. #AP9888c) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q8IZD4
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 67723 Da
Antigen Region 261-290 aa
Additional Information
Gene ID 196513
Other Names mRNA-decapping enzyme 1B, 3---, DCP1B
Target/Specificity This DCP1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 261-290 amino acids of human DCP1B.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDCP1B Antibody (center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DCP1B
Function May play a role in the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. May remove the 7- methyl guanine cap structure from mRNA molecules, yielding a 5'- phosphorylated mRNA fragment and 7m-GDP (By similarity).
Cellular Location Cytoplasm. Nucleus {ECO:0000250|UniProtKB:Q9NPI6}
Citations (0)
citation

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Background

DCP1B is a core component of the mRNA decapping complex, a key factor in the regulation of mRNA decay.

References

Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)
Lehner, B., et al. Genome Res. 14(7):1315-1323(2004)
Cougot, N., et al. J. Cell Biol. 165(1):31-40(2004)

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$ 182.50
$ 70.00
Cat# AP9888c
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