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PYCR1 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | P32322 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 33361 Da |
| Antigen Region | 291-319 aa |
| Gene ID | 5831 |
|---|---|
| Other Names | Pyrroline-5-carboxylate reductase 1, mitochondrial, P5C reductase 1, P5CR 1, PYCR1 |
| Target/Specificity | This PYCR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-319 amino acids from the C-terminal region of human PYCR1. |
| Dilution | FC~~1:10~50 IHC-P~~1:50~100 WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PYCR1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PYCR1 (HGNC:9721) |
|---|---|
| Function | Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5- carboxylate to L-proline using NAD(P)H (PubMed:16730026, PubMed:19648921, PubMed:23024808, PubMed:28258219). At physiologic concentrations, has higher specific activity in the presence of NADH (PubMed:16730026, PubMed:23024808). Involved in the cellular response to oxidative stress (PubMed:16730026, PubMed:19648921). |
| Cellular Location | Mitochondrion |
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion.
References
Reversade, B., et al. Nat. Genet. 41(9):1016-1021(2009)
Guernsey, D.L., et al. Am. J. Hum. Genet. 85(1):120-129(2009)
Meng, Z., et al. J. Mol. Biol. 359(5):1364-1377(2006)
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