NOL9 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application
| FC, WB, E |
---|---|
Primary Accession | Q5SY16 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 79323 Da |
Antigen Region | 668-696 aa |
Gene ID | 79707 |
---|---|
Other Names | Polynucleotide 5'-hydroxyl-kinase NOL9, 271-, Nucleolar protein 9, NOL9 |
Target/Specificity | This NOL9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 668-696 amino acids from the C-terminal region of human NOL9. |
Dilution | WB~~1:1000 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | NOL9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NOL9 |
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Function | Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of single-stranded and double-stranded RNA and DNA substrates (PubMed:21063389). Involved in rRNA processing and its kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form (PubMed:21063389). Required for the efficient pre-rRNA processing of internal transcribed spacer 2 (ITS2) (PubMed:21063389). Associates with LAS1L to form an ITS2 pre-rRNA endonuclease-kinase complex and is responsible for the transport of this complex into the nucleolus (PubMed:31288032). |
Cellular Location | Nucleus {ECO:0000250|UniProtKB:Q3TZX8}. Nucleus, nucleolus. Note=Colocalizes with pre-60S rRNP particles |
Provided below are standard protocols that you may find useful for product applications.
References
Scherl, A., et al. Mol. Biol. Cell 13(11):4100-4109(2002) Wistow, G., et al. Mol. Vis. 8, 205-220 (2002) Andersen, J.S., et al. Curr. Biol. 12(1):1-11(2002)
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