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NOL9 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NOL9 Antibody (C-term) AP9913b
    NOL9 Antibody (C-term) (Cat. #AP9913b) western blot analysis in Jurkat,293,Hela cell line lysates (35ug/lane).This demonstrates the NOL9 antibody detected the NOL9 protein (arrow).
    detail
  • FC - NOL9 Antibody (C-term) AP9913b
    NOL9 Antibody (C-term) (Cat. #AP9913b) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, E
Primary Accession Q5SY16
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 79323 Da
Antigen Region 668-696 aa
Additional Information
Gene ID 79707
Other Names Polynucleotide 5'-hydroxyl-kinase NOL9, 271-, Nucleolar protein 9, NOL9
Target/Specificity This NOL9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 668-696 amino acids from the C-terminal region of human NOL9.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNOL9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NOL9
Function Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of single-stranded and double-stranded RNA and DNA substrates (PubMed:21063389). Involved in rRNA processing and its kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form (PubMed:21063389). Required for the efficient pre-rRNA processing of internal transcribed spacer 2 (ITS2) (PubMed:21063389). Associates with LAS1L to form an ITS2 pre-rRNA endonuclease-kinase complex and is responsible for the transport of this complex into the nucleolus (PubMed:31288032).
Cellular Location Nucleus {ECO:0000250|UniProtKB:Q3TZX8}. Nucleus, nucleolus. Note=Colocalizes with pre-60S rRNP particles
Research Areas
Citations ( 0 )

References

Scherl, A., et al. Mol. Biol. Cell 13(11):4100-4109(2002) Wistow, G., et al. Mol. Vis. 8, 205-220 (2002) Andersen, J.S., et al. Curr. Biol. 12(1):1-11(2002)

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$ 365.00
$ 140.00
Cat# AP9913b
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