DEN1A Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| FC, IHC-P, WB, E |
---|---|
Primary Accession | Q8TEH3 |
Other Accession | Q8K382 |
Reactivity | Human |
Predicted | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 110577 Da |
Antigen Region | 576-605 aa |
Gene ID | 57706 |
---|---|
Other Names | DENN domain-containing protein 1A, Connecdenn 1, Connecdenn, Protein FAM31A, DENND1A, FAM31A, KIAA1608 |
Target/Specificity | This DEN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-605 amino acids from the C-terminal region of human DEN1A. |
Dilution | WB~~1:500 IHC-P~~1:50~100 FC~~1:10~50 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | DEN1A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | DENND1A (HGNC:29324) |
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Function | Guanine nucleotide exchange factor (GEF) regulating clathrin- mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP- bound form. Regulates clathrin-mediated endocytosis of synaptic vesicles and mediates exit from early endosomes (PubMed:20154091, PubMed:20937701). Binds phosphatidylinositol-phosphates (PtdInsPs), with some preference for PtdIns(3)P (By similarity). |
Cellular Location | Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein. Presynaptic cell membrane. Note=Associates to membranes via lipid-binding activity. {ECO:0000250|UniProtKB:Q8K382} |
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Provided below are standard protocols that you may find useful for product applications.
References
Allaire, P.D., et al. J. Neurosci. 26(51):13202-13212(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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