INT10 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| FC, WB, E |
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Primary Accession | Q9NVR2 |
Other Accession | Q4R7B1 |
Reactivity | Human |
Predicted | Monkey |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 82236 Da |
Antigen Region | 516-545 aa |
Gene ID | 55174 |
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Other Names | Integrator complex subunit 10, Int10, INTS10, C8orf35 |
Target/Specificity | This INT10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human INT10. |
Dilution | FC~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration. |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | INT10 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | INTS10 {ECO:0000303|PubMed:38823386, ECO:0000312|HGNC:HGNC:25548} |
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Function | Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes (PubMed:38570683, PubMed:38823386). The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA (PubMed:38570683). The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs) (PubMed:16239144, PubMed:32647223). Within the integrator complex, INTS10 is part of the integrator tail module that acts as a platform for the recruitment of transcription factors at promoters (PubMed:38823386). May be not involved in the recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the integrator complex (PubMed:23904267). |
Cellular Location | Nucleus |

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Provided below are standard protocols that you may find useful for product applications.
Background
INTS10 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1.
References
Lasky-Su, J., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 147B (8), 1345-1354 (2008)
Kooner, J.S., et al. Nat. Genet. 40(2):149-151(2008)
Baillat, D., et al. Cell 123(2):265-276(2005)
Yuryev, A., et al. Genomics 81(2):112-125(2003)

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