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Smac Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E, IP |
|---|---|
| Primary Accession | Q9JIQ3 |
| Other Accession | NP_063940, 66593 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 26 kDa Observed: 25 kDa |
| Application Notes | Smac antibody can be used for detection of Smac/DIABLO by Western blot at 1 μg/mL. Smac antibody can also be used to detect Smac/DIABLO by immunohistochemistry at 5 μg/mL. |
| Gene ID | 66593 |
|---|---|
| Other Names | Smac Antibody: Smac, AU040403, 0610041G12Rik, 1700006L01Rik, Smac, Diablo homolog, mitochondrial, Direct IAP-binding protein with low pI, diablo homolog (Drosophila) |
| Target/Specificity | Smac antibody was raised against a 15 amino acid peptide near the carboxy terminus of human Smac. The immunogen is located within the last 50 amino acids of Smac. |
| Reconstitution & Storage | Smac antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | Smac Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Diablo {ECO:0000312|MGI:MGI:1913843} |
|---|---|
| Function | Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases (By similarity). |
| Cellular Location | Mitochondrion. Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9NR28}. Note=Released into the cytosol in a PARL-dependent manner when cells undergo apoptosis {ECO:0000250|UniProtKB:Q9NR28} |
| Tissue Location | Highest expression found in heart, liver, kidney and testis. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Smac Antibody: The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.
References
Du C, Fang M, Li Y, et al. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition. Cell 2000; 102:33-42.
Verhagen AM, Ekert PG, Pakusch M, et al. Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins. Cell 2000;102:43-53.
Srinivasula SM, Datta P, Fan XJ, et al. Molecular Determinants of the Caspase-promoting Activity of Smac/DIABLO and Its Role in the Death Receptor Pathway. J. Biol. Chem. 2000; 275:36152-7.
Chai J, Du C, Wu JW, et al. Structural and biochemical basis of apoptotic activation by Smac/DIABLO. Nature 2000; 406:855-62.
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