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BCAS3 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q9H6U6 |
| Other Accession | EAW51415, 119571800 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 101237 Da |
| Application Notes | BCAS3 antibody can be used for detection of BCAS3 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 54828 |
|---|---|
| Target/Specificity | BCAS3; |
| Reconstitution & Storage | BCAS3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | BCAS3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BCAS3 {ECO:0000312|HGNC:HGNC:14347, ECO:0000312|MIM:607470} |
|---|---|
| Function | Plays a role in angiogenesis. Participates in the regulation of cell polarity and directional endothelial cell migration by mediating both the activation and recruitment of CDC42 and the reorganization of the actin cytoskeleton at the cell leading edge. Promotes filipodia formation (By similarity). Functions synergistically with PELP1 as a transcriptional coactivator of estrogen receptor- responsive genes. Stimulates histone acetyltransferase activity. Binds to chromatin. Plays a regulatory role in autophagic activity. In complex with PHAF1, associates with the preautophagosomal structure during both non-selective and selective autophagy (PubMed:33499712). Probably binds phosphatidylinositol 3-phosphate (PtdIns3P) which would mediate the recruitment preautophagosomal structures (PubMed:33499712). |
| Cellular Location | Nucleus. Cytoplasm. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:Q8CCN5}. Preautophagosomal structure. Note=Localizes in the cytoplasm in stationary cells. Translocates from the cytoplasm to the leading edge in motile cells. Colocalizes with microtubules and intermediate filaments in both stationary and motile cells (By similarity) Associates with chromatin. Recruited to estrogen receptor-induced promoters in a PELP1-dependent manner. The BCAS3:PHAF1 complex is recruited to the preautophagosomal structures adjacent to the damaged mitochondria upon mitophagy in a PRKN-PINK1 dependent manner (PubMed:33499712). {ECO:0000250|UniProtKB:Q8CCN5, ECO:0000269|PubMed:17505058, ECO:0000269|PubMed:33499712} |
| Tissue Location | Expressed in stomach, liver, lung, kidney, prostate, testis, thyroid gland, adrenal gland, brain, heart, skeletal muscle, colon, spleen, small intestine, placenta, blood leukocyte and mammary epithelial cells. Expressed in undifferentiated ES cells Expressed in blood islands and nascent blood vessels derived from differentiated ES cells into embryoid bodies (BD). Expressed in endothelial cells. Not detected in brain. Expressed in brain tumors (at protein level). Expressed in brain. Highly expressed in breast cancers and in glioma cell lines. |
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Provided below are standard protocols that you may find useful for product applications.
Background
BCAS3 Antibody: The Breast carcinoma amplified sequence (BCAS3) gene is localized to 17q23, a region that is often amplified in breast cancers. Copy number gains at this region have also been reported in other tumors such as brain, lung, liver, testis and bladder. BCAS3 is thought to be an estrogen receptor (ER)-alpha co-activator that acts through PELP1, another ER-alpha that in turn activates BCAS3 expression. This may set up a positive feedback loop leading to ER-a signal amplification in the cell which may play a significant role in breast cancer. BCAS3 expression has also been observed in embryonic stem cells and vascular precursors, suggesting that it may also play a role in embryogenesis and tumor angiogenesis.
References
Barlund M, Monni O, Weaver JD, et al. Cloning of BCAS3 (17q23) and BCAS4 (20q13) genes that undergo amplification, overexpression, and fusion in breast cancer. Genes Chromosomes Cancer2002; 35:311-7.
Sinclair CS, Rowley M, Naderi A, et al. The 17q23 amplicon and breast cancer. Breast Cancer Res. Treat.2003; 78:313-22.
Gururaj AE, Peng S, Vadlamudi RK, et al. Estrogen induces expression of BCAS3, a novel estrogen receptor-a coactivator, through Proline-, Glutamic acid-, and leucine-rich protein -1 (PELP1). Mol. Endocrinol.2007; 21:1847-60.
Siva K, Venu P, Mahadevan A, et al. Human BCAS3 expression in embryonic stem cells and vascular precursors suggests a role,in human ebryogenesis and tumor angiogenesis. PloS One2007; 11:e1202.
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