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EFHD1 Antibody

     
  • IF - EFHD1 Antibody ASC11089
    Immunofluorescence of EFHD1 in rat spleen tissue with EFHD1 antibody at 5 µg/mL.
    detail
  • IF - EFHD1 Antibody ASC11089
    Immunofluorescence of EFHD1 in Rat Spleen cells with EFHD1 antibody at 20 µg/mL.
    detail
  • WB - EFHD1 Antibody ASC11089
    Western blot analysis of EFHD1 in human spleen tissue lysate with EFHD1 antibody at (A) 2 and (B) 4 µg/mL.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q9BUP0
Other Accession NP_079478, 20149496
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW 26928 Da
Application Notes EFHD1 antibody can be used for detection of EFHD1 by Western blot at 2 - 4 µg/mL. Antibody can also be used for immunoflourescence starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 80303
Target/Specificity EFHD1;
Reconstitution & Storage EFHD1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsEFHD1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EFHD1
Synonyms SWS2
Function Acts as a calcium sensor for mitochondrial flash (mitoflash) activation, an event characterized by stochastic bursts of superoxide production (PubMed:26975899). May play a role in neuronal differentiation (By similarity).
Cellular Location Mitochondrion inner membrane {ECO:0000250|UniProtKB:Q9D4J1}
Research Areas
Citations (0)
citation

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Background

EFHD1 Antibody: EFHD1, also known as Swiprosin-2 or SWS2, is an EF-hand and coiled-coil-containing adaptor protein identified in a subtractive hybridization study using a neuronal cell line established from the cerebellum of an adult p53-null mouse, however further study indicated no difference in normal mice. Its mRNA is widely expressed, with its expression in brain undetectable at embryonic stages, with increasing levels from postnatal to adult development. In situ hybridization showed expression in neurons but not white matter of the cerebellum and cerebrum. EHFD1 is also highly expressed in testes, ovary, and the collecting ducts of the kidney, suggesting that in non-neuronal cells, EFHD1 may be involved in gametogenesis and water-reabsorbtion.

References

Tominaga M and Tomooka Y. Novel genes cloned from a neuronal cell line newly established from a cerebellum of an adult p53-/- mouse. Biochem. Biophys. Res. Comm.2002; 297:473-9.


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$ 379.00
Cat# ASC11089
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