Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
REQUIEM Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q92785 |
| Other Accession | AAB81203, 5454004 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 44155 Da |
| Application Notes | REQUIEM antibody can be used for detection of REQUIEM by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 5977 |
|---|---|
| Target/Specificity | DPF2; Requiem antibody is predicted to not cross-react with other DPF protein family members. |
| Reconstitution & Storage | REQUIEM antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | REQUIEM Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DPF2 |
|---|---|
| Synonyms | BAF45D, REQ, UBID4 |
| Function | Plays an active role in transcriptional regulation by binding modified histones H3 and H4 (PubMed:28533407, PubMed:27775714). Is a negative regulator of myeloid differentiation of hematopoietic progenitor cells (PubMed:28533407). Might also have a role in the development and maturation of lymphoid cells (By similarity). Involved in the regulation of non-canonical NF-kappa-B pathway (PubMed:20460684). |
| Cellular Location | Nucleus. Cytoplasm |
| Tissue Location | Ubiquitous. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
REQUIEM Antibody: Requiem is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It is expressed in numerous tissues in both cytosolic and nuclear regions and likely serves a regulatory role in rapid hematopoietic cell growth and turnover. Requiem has been found to act as an adaptor protein that links the NF-κB and SWI/SNF chromatin remodeling factor and is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.
References
Gabig TG, Mantel PL, Rosli R, et al. Requiem: a novel zinc finger gene essential for apoptosis in myeloid cells. J. Biol. Chem.1994; 269:29515-9.
Gabig TG, Crean CD, Klenk A, et al. Expression and chromosomal localization of the Requiem gene. Mamm. Genome1998; 9:660-5.
Tando T, Ishizaka A, Watanable H, et al. Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway. J. Biol. Chem.2010; 285:21951-60.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
