HDGFRP2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
---|---|
Primary Accession | Q7Z4V5 |
Other Accession | EAW69215, 48255931 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 74317 Da |
Application Notes | HDGFRP2 antibody can be used for detection of HDGFRP2 by Western blot at 0.5 - 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
Gene ID | 84717 |
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Target/Specificity | HDGFRP2; At least two isoforms of HDGFRP2 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other HDGFRP family members |
Reconstitution & Storage | HDGFRP2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | HDGFRP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | HDGFL2 (HGNC:14680) |
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Function | Acts as an epigenetic regulator of myogenesis in cooperation with DPF3a (isoform 2 of DPF3/BAF45C) (PubMed:32459350). Associates with the BAF complex via its interaction with DPF3a and HDGFL2-DPF3a activate myogenic genes by increasing chromatin accessibility through recruitment of SMARCA4/BRG1/BAF190A (ATPase subunit of the BAF complex) to myogenic gene promoters (PubMed:32459350). Promotes the repair of DNA double-strand breaks (DSBs) through the homologous recombination pathway by facilitating the recruitment of the DNA endonuclease RBBP8 to the DSBs (PubMed:26721387). Preferentially binds to chromatin regions marked by H3K9me3, H3K27me3 and H3K36me2 (PubMed:26721387, PubMed:32459350). Involved in cellular growth control, through the regulation of cyclin D1 expression (PubMed:25689719). |
Cellular Location | Nucleus. Cytoplasm {ECO:0000250|UniProtKB:Q925G1} |
Tissue Location | Widely expressed. High expression is found in heart, skeletal muscle, ovary and testis. Overexpression is frequently observed in hepatocellular carcinoma samples |
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Provided below are standard protocols that you may find useful for product applications.
Background
HDGFRP2 Antibody: Hepatoma-derived growth factor-related protein 2 (HDGFRP2) is a widely expressed member of protein family that contains the HDGF related proteins 1-4 and lens epithelial derived growth factor (LEDGF). These family members show extensive homology at the amino terminus (termed the hath region). Little is known of the role of HDGFRP2, but like the related LEDGF, HDGFRP2 can bind HIV-1 integrase and stimulate HIV-1 integrase activity in vitro.
References
El-Tahir HM, Dietz F, Dringen R, et al. Expression of Hepatoma-derived growth factor family members in the adult central nervous system. BMC Neurosci. 2006; 7:6.
Izumoto Y, Kuroda T, Harada H, et al. Hepatoma-derived growth factor belongs to a gene family in mice showing significant homology in the amino terminus. Biochem. Biophys. Res. Commun. 1997; 238:26-32
Cherepanov P, Devroe E, Silver PA, et al. Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase. J. Biol. Chem. 2004; 279:48883-92.
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