HHATL Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
---|---|
Primary Accession | Q9HCP6 |
Other Accession | NP_065758, 239582769 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 56703 Da |
Application Notes | HHATL antibody can be used for detection of HHATL by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 2.5 µg/mL. |
Gene ID | 57467 |
---|---|
Target/Specificity | HHATL; |
Reconstitution & Storage | HHATL antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | HHATL Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | HHATL |
---|---|
Synonyms | C3orf3, GUP1, KIAA1173 |
Function | Negatively regulates N-terminal palmitoylation of SHH by HHAT/SKN. |
Cellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein |
Tissue Location | Heart-specific.. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
HHATL Antibody: The Protein-cysteine N-palmitoyltransferase HHAT-like protein (HHATL), also known at the mammalian glycerol uptake/transporter 1 (Gup1) is a homolog of the S. cerevisiae Gup1. It is an endoplasmic reticulum (ER) membrane protein that co-localizes with the mammalian hedgehog acyltransferse Skn and interferes with its N-terminal palmitoylation of Sonic hedgehog (SHH), suggesting that HHATL acts as a negative regulator for N-terminal palmitoylation of SHH. HHATL is highly expressed in normal skin, but becomes down-regulated in skin squamous cell carcinoma, suggesting it may play a role in its development.
References
Abe Y, Kita Y, and Niikura T. Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-palmitoylation of Sonic hedgehog. FEBS J. 2008; 275:318-31.
Zhang SQ, Tian X, Luo YW, et al. Expression, clinical and pathological significance of KIAA1173 gene in skin squamous cell carcinoma. Zhonghua Yi Xue Za Zhi 2010; 90:1243-6.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.