NGN2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF, E |
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Primary Accession | Q9H2A3 |
Other Accession | NP_076924, 31077092 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 30 kDa |
Application Notes | NGN2 antibody can be used for detection of NGN2 by Western blot at 1 - 2 µg/mL. For immunofluorescence start at 20 µg/mL. |
Gene ID | 63973 |
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Target/Specificity | NEUROG2; NGN2 antibody is predicted to not cross-react with neurogenin 1. |
Reconstitution & Storage | NGN2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | NGN2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NEUROG2 |
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Synonyms | ATOH4, BHLHA8, NGN2 |
Function | Transcriptional regulator. Involved in neuronal differentiation. Activates transcription by binding to the E box (5'- CANNTG-3'). |
Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}. |
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Provided below are standard protocols that you may find useful for product applications.
Background
NGN2 Antibody: Neurogenin-2 (NGN2) is a neural-specific basic helix-loop-helix (bHLH) transcription factor that can specify a neuronal fate on ectodermal cells and is expressed in neural progenitor cells within the developing central and peripheral nervous systems. NGN2 is thought to work with Nurr1 to play a role in the differentiation and survival of midbrain dopaminergic neurons. It has also been suggested for use in human embryonic neural progenitors as a graft for spinal cord injuries
References
Sommer L, Ma Q, and Anderson DJ. Neurogenins, a novel family of atonal-related bHLH transcription factors, a putative mammalian neuronal determination genes that reveal progenitor cell heterogeneity in the developing CNS and PNS. Mol. Cell Neurosci. 1996; 8:221-41.
Fode C, Gradwohl G, Morin X, et al. The bHLH protein NEUROGENIN 2 is a determination factor for epibranchial placode-derived sensory neurons. Neuron 1998; 20:483-94.
Andersson EK, Irvin DK, Ahlsio J, et al. Ngn2 and Nurr1 act in synergy to induce midbrain dopaminergic neurons from expanded neural stem and progenitor cells. Exp. Cell. Res. 2007; 313:1172-80.
Perrin FE, Boniface G, Serguera C, et al. Grafted human embryonic progenitors expressing neurogenin-2 stimulate axonal sprouting and improve motor recovery after severe spinal cord injury. PLoS One 2010; 5:e15914.
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