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APPBP2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | Q92624 |
| Other Accession | NP_006371, 18104962 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 64 kDa Observed: 57 kDa |
| Application Notes | APPBP2 antibody can be used for detection of APPBP2 by Western blot at 0.5 - 1 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 10513 |
|---|---|
| Target/Specificity | APPBP2; APPBP2 antibody is human, mouse and rat reactive. At least two isoforms of APPBP2 are known to exist; this antibody will detect both isoforms. |
| Reconstitution & Storage | APPBP2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | APPBP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | APPBP2 {ECO:0000303|PubMed:26138980, ECO:0000312|HGNC:HGNC:622} |
|---|---|
| Function | Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:29779948, PubMed:29775578). The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms (PubMed:29779948, PubMed:29775578). The CRL2(APPBP2) complex specifically recognizes proteins with a -Arg-Xaa- Xaa-Gly degron at the C-terminus, leading to their ubiquitination and degradation (PubMed:29779948, PubMed:29775578). The CRL2(APPBP2) complex mediates ubiquitination and degradation of truncated SELENOV selenoproteins produced by failed UGA/Sec decoding, which end with a -Arg-Xaa-Xaa-Gly degron (PubMed:26138980). May play a role in intracellular protein transport: may be involved in the translocation of APP along microtubules toward the cell surface (PubMed:9843960). |
| Cellular Location | Nucleus. Cytoplasm, cytoskeleton. Membrane; Peripheral membrane protein. Note=Associated with membranes and microtubules. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The amyloid beta precursor protein (cytoplasmic tail) binding protein 2 (APPBP2), also known as PAT1, interacts with microtubules and is functionally associated with beta-amyloid precursor protein transport and/or processing (1). The beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease (2). APPBP2 has been found to be highly expressed in breast cancer (3).
References
Zheng P, Eastman J, Vande Pol S, et al. PAT1, a microtubule-interacting protein, recognizes the basolateral sorting signal of amyloid precursor protein. Proc. Natl. Acad. Sci. USA 1994; 95:14745-50.
Selkoe DJ. Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease. Annu. Rev. Cell Biol. 1994; 10:373-403.
Li J, Yang Y, Peng Y, et al. Oncogenic properties of PPM1D located within a breast cancer amplification epicenter at 17q23. Nat. Genet. 2002; 31:133-4.
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