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IL-22 Antibody

     
  • WB - IL-22 Antibody ASC11732
    Western blot analysis of IL-22 in HeLa cell lysate with IL-22 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
    detail
  • ICC - IL-22 Antibody ASC11732
    Immunocytochemistry of IL-22 in HeLa cells with IL-22 antibody at 5 µg/mL.
    detail
  • IF - IL-22 Antibody ASC11732
    Immunofluorescence of IL-22 in HeLa cells with IL-22 antibody at 20 µg/mL.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, ICC, E
Primary Accession Q9GZX6
Other Accession NP_065386, 10092625
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 20 kDa

Observed: 25 kDa
Application Notes IL-22 antibody can be used for detection of IL-22 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 50616
Target/Specificity IL22; IL-22 antibody is human, mouse, and rat reactive. Multiple isoforms of IL-22 are known to exist.
Reconstitution & Storage IL-22 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsIL-22 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL22
Synonyms ILTIF, ZCYTO18
Function Cytokine that plays a critical role in modulating tissue responses during inflammation (PubMed:17204547). Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage (PubMed:17204547). Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB (PubMed:10875937, PubMed:18599299). Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3. In turn, promotes cell survival and proliferation through STAT3, ERK1/2 and PI3K/AKT pathways (PubMed:25793261, PubMed:31311100). Promotes phosphorylation of GSK3B at 'Ser-9' and CTTN (By similarity). Promotes epithelial cell spreading (By similarity).
Cellular Location Secreted.
Research Areas
Citations (0)
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Background

Interleukin-22 (IL-22) is a cytokine important for the modulation of tissue responses during inflammation (1). Unlike the distantly related IL-10, IL-22 does not inhibit the production of proinflammatory cytokines in monocytes in response to LPS, but it has some inhibitory effects on IL-4 production from Th2 T cells. IL-22 is expressed by both the adaptive arm of the immune system such as CD4 T cell subsets including Th17 cells, as well as by innate lymphocytes such as NK and LTi-like cells (2). IL-22 is highly expressed in several chronic inflammatory conditions, and studies suggest that IL-22 plays both inflammatory and protective roles (3).

References

Xie MH, Aggarwal S, Ho WH, et al. Interleukin (IL)-22, a novel human cytokine that signals through the interferon receptor-related proteins CRF2-4 and IL-22R. J. Biol. Chem. 2000; 275:31335-9.
Wolk K, Kunz S, Witte E, et al. IL-22 increases the innate immunity of tissues. Immunity 2004; 21:241-54.
Wolk K, Witte E, Wallace E, et al. IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis. Eur. J. Immunol. 36:1309-23.

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$ 379.00
Cat# ASC11732
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