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DOCK8 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q8NF50 |
| Other Accession | NP_982272, 238231392 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 166, 220, 231 kDa Observed: 220, 230 kDa |
| Application Notes | DOCK8 antibody can be used for detection of DOCK8 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. |
| Gene ID | 81704 |
|---|---|
| Target/Specificity | DOCK8; DOCK8 antibody is human, mouse and rat reactive. Multiple isoforms of DOCK8 are known to exist. |
| Reconstitution & Storage | DOCK8 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | DOCK8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DOCK8 |
|---|---|
| Function | Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed:28028151, PubMed:22461490). During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (By similarity). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed:28028151). Is involved in NK cell cytotoxicity by controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (PubMed:25762780). |
| Cellular Location | Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Note=Enriched and co-localizes with GTPase CDC42 at the immunological synapse formed during T cell/antigen presenting cell cognate interaction. Translocates from the cytoplasm to the plasma membrane in response to chemokine CXCL12/SDF-1-alpha stimulation |
| Tissue Location | Expressed in peripheral blood mononuclear cells (PBMCs). |
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Provided below are standard protocols that you may find useful for product applications.
Background
The Dedicator of cytokinesis protein 8 (DOCK8) is a member of the DOCK180 family of guanine nucleotide exchange factors (1). DOCK8 plays an essential role in humoral immune responses and is important in the proper formation of the B cell immunological synapse (reviewed in 2). Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome (3).
References
Ruusala A and Aspenstrom P. Isolation and characterisation of DOCK8, a member of the DOCK180-related regulators of cell morphology. FEBS Lett. 2004; 572:159-66.
Randall KL, Lambe T, Goodnow CC, et al. The essential role of DOCK8 in humoral immunity. Dis. Markers 2010; 29:141-50.
Engelhardt KR, McGhee S, Sinkler S, et al. Large deletions and point mutations involving the dedicator of cytokinesis 8 (DOCK8) in the autosomal recessive form of hyper-IgE syndrome. J. Allergy Clin. Immunol. 2009; 124:1289-302.
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