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CD86 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | P42082 |
| Other Accession | NP_787058 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone Names | CD86 |
| Calculated MW | Predicted: 36 kDa Observed: 68 kDa |
| Gene ID | 942 |
|---|---|
| Alias Symbol | CD86 |
| Other Names | CD86 Antibody: CD86 molecule, B70, B7-2, B7.2, LAB72, CD28LG2 |
| Target/Specificity | At least five isoforms of CD86 are known to exist; this antibody will detect all five isoforms. |
| Reconstitution & Storage | CD86 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | CD86 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Cd86 |
|---|---|
| Function | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (PubMed:23241883). |
| Cellular Location | Cell membrane; Single-pass type I membrane protein |
| Tissue Location | Expressed on activated B-cells. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CD86, also known as B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily. Like the related protein CD80, this protein is expressed by antigen-presenting cells, and is the ligand for two proteins at the cell surface of T cells, CD28 and the cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production. CTLA-4 binding negatively regulates T-cell activation and diminishes the immune response (1). Blocking the CTLA-4-CD80/CD86 interaction has been shown to enhance T-cell functions in acute lymphoblastomic leukemia (ALL), suggesting that this pathway may be an attractive target for future cancer immunotherapy (2).
References
Lane P. Regulation of T and B cell responses by modulating interactions between CD28/CTLA-4 and their ligands, CD80 and CD86. Ann NY Acad Sci 1997; 815:392-400.Feucht J, Kayser S, Gorodezki D, et al. T-cell responses against CD19+ pediatric acute lymphoblastic leukemia mediated by bispecific T-cell engager (BiTE) are regulated contrarily by PD-L1 and CD80/CD86 on leukemic blasts. Oncotarget 2016; 7:76902-19.
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