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SARS-CoV-2 (COVID-19) ORF3b Antibody
Infectious Disease, COVID-19
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, E |
|---|---|
| Primary Accession | P0DTC5 |
| Other Accession | P0DTC3 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone Names | ORF3b |
| Calculated MW | 25147 Da |
| Application Notes | WB: 0.25 μg/mL; IHC: 0.5 μg/mL Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) ORF3b antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 ORF3b recombinant protein by ELISA and WB. All other applications and species not yet tested. |
| Gene ID | 43740569 |
|---|---|
| Other Names | ORF3b protein, Acessary protein 3b, ns3b, Non-structural protein 3b, ORF3b |
| Target/Specificity | ORF3b Antibody is predicted to not cross-react with other coronavirus family members. |
| Reconstitution & Storage | SARS-CoV-2 (COVID-19) ORF3b antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | SARS-CoV-2 (COVID-19) ORF3b Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | M |
|---|---|
| Function | Component of the viral envelope that plays a central role in virus morphogenesis and assembly via its interactions with other viral proteins (By similarity). Regulates the localization of S protein at cis-Golgi, the place of virus budding (PubMed:33229438). May act by binding cytoplasmic c-terminus of S (PubMed:33229438). |
| Cellular Location | Virion membrane {ECO:0000255|HAMAP- Rule:MF_04202}; Multi-pass membrane protein {ECO:0000255|HAMAP- Rule:MF_04202}. Host Golgi apparatus membrane {ECO:0000255|HAMAP- Rule:MF_04202, ECO:0000269|PubMed:33060197}; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_04202}. Host membrane; Multi-pass membrane protein Note=Largely embedded in the lipid bilayer. {ECO:0000255|HAMAP- Rule:MF_04202} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF3b, ORF9b, ORF9c and ORF10. ORF3b may play a role in interferon antiviral system evasion (3).
References
Gorbalenya. bioRxiv: 2020.;Hui et al. Int J Infect Dis. 2020;91:264-266.;Konno et al. Cell Rep. 2020; 32:108185.
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