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SARS-CoV-2 (COVID-19) ORF8 Antibody
Infectious Disease, COVID-19
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, E |
|---|---|
| Primary Accession | P0DTC8 |
| Other Accession | P0DTC8 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Clone Names | ORF8 |
| Calculated MW | 13831 Da |
| Application Notes | WB: 1 μg/mL; IHC: 0.1 μg/mL Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) ORF8 antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 ORF8 recombinant protein by ELISA and WB. All other applications and species not yet tested. |
| Gene ID | 43740577 |
|---|---|
| Other Names | ORF8 protein, ns8, ORF8, Non-structural protein 8 |
| Target/Specificity | ORF8 Antibody is predicted to not cross-react with other coronavirus family members. |
| Reconstitution & Storage | SARS-CoV-2 (COVID-19) ORF8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | SARS-CoV-2 (COVID-19) ORF8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | 8 |
|---|---|
| Function | Plays a role in modulating the host immune response (PubMed:31986261, PubMed:35343786, PubMed:36689483). May act as a secreted virokine by mimicking interleukin-17A (IL17A), and thereby binding to the IL17RA receptor, leading to activation of the IL17 pathway and increased secretion of pro-inflammatory factors (PubMed:35343786, PubMed:36689483). Contributes to the cytokine storm during SARS-CoV-2 infection when secreted by unconventional pathway (PubMed:33723527, PubMed:36689483). May act by down-regulating major histocompability complex class I (MHC-I) at cell surface (PubMed:34021074, PubMed:35157849). May inhibit expression of some members of the IFN-stimulated gene (ISG) family including hosts IGF2BP1/ZBP1, MX1 and MX2, and DHX58 (PubMed:34177923). |
| Cellular Location | Secreted. Note=Is secreted during a normal viral infection by unconventional pathway (PubMed:35157849, PubMed:36689483) Its mRNA is expressed in cytoplasm and not spliced during a viral infection, but is spliced when expressed from cDNA in nucleus (PubMed:35157849). Splicing changes localization to host endosome and/or cytoplasm (PubMed:33060197, PubMed:34177923). May also localize in nucleus when fused with GFP (PubMed:34177923) |
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Background
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF9c and ORF10. ORF8 may play a role in modulating host immune response (Probable). May play a role in blocking host IL17 cytokine by its interaction with host IL17RA (3).
References
Gorbalenya. bioRxiv: 2020.;Hui et al. Int J Infect Dis. 2020;91:264-266.;Chan et al. Lancet. 2020; 395:514-523.
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