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HIF2 alpha Antibody
HIF 2 alpha Antibody, Clone EP190B
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, ICC, E, AM |
|---|---|
| Primary Accession | Q99814 |
| Other Accession | NP_001421.4 |
| Host | Mouse |
| Isotype | IgG1 |
| Reactivity | Human, Mouse |
| Clonality | Monoclonal |
| Description | Mouse Anti-Human HIF2 alpha Monoclonal IgG1 |
| Target/Specificity | Detects ~100kDa. Specific for HIF2 Alpha. |
| Other Names | ECYT4 Antibody, EPAS1 Antibody, HIF2alpha Antibody, HIF1 Alpha like factor Antibody, HLF Antibody, Hypoxia inducible factor 2 alpha Antibody, MOP2 Antibody, PASD2 Antibody |
| Clone Names | EP190B |
| Immunogen | GST-human EPAS-1 (HIF2alpha) amino acids 535-631 fusion protein |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 1 µg/ml of SMC-185 was sufficient for detection of HIF2α in 20 µg of CoCl2-induced Hela cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Nucleus |
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Provided below are standard protocols that you may find useful for product applications.
Background
Members of the hypoxia-inducible factor (HIF) family of transcription factors regulate the cellular response to hypoxia (1). HIF2-Alpha is involved in catecholamine homeostasis, vascular remodeling, physiological angiogenesis and adipogenesis. It is overexpressed in many cancerous tissues, but its exact role in tumourprogression remains to be clarified (2). Studies suggest that in the case of non-small cell lung cancer, HIF2-Alpha actually is a promoter of tumor growth and progression in a solid tumor (1). Other data suggests that HIF2-Alpha is an important regulator of innate immunity, and therefore may be useful in the therapeutic target for treating inflammatory disorders and cancer (3).
References
1. Kim W.Y., et al. (2009) J Clin Invest. 119(8): 2160-2170.
2. Favier J., Lapointe S., Maliba R. and Sirois M.G. (2007) BMC Cancer. 7:139.
3. Imtiyaz H.Z., et al. (2010) J Clin Invest. 120(8): 2699-2714.
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