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CENP-A Antibody
CENP-A Antibody, Clone 5A7-2E11
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC |
|---|---|
| Primary Accession | P49450 |
| Other Accession | NP_001035891.1 |
| Host | Mouse |
| Isotype | IgG1 |
| Reactivity | Human |
| Clonality | Monoclonal |
| Description | Mouse Anti-Human CENP-A Monoclonal IgG1 |
| Target/Specificity | Detects ~18kDa. Recognizes Human CENP-A. |
| Other Names | CENP A Antibody, cenpa Antibody, Centromere auto antigen A Antibody, Histone H3 like centromeric protein A Antibody |
| Clone Names | 5A7-2E11 |
| Immunogen | Synthetic peptide corresponding to a portion of human CENP-A |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 1 µg/ml of SMC-202 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Nucleus | Chromosome | Centromere | Kinetochore |
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Provided below are standard protocols that you may find useful for product applications.
Background
A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The Centromere Protein-A, CENP-A, is a Histone H3-like protein that contains a C-terminal H3-like domain, which is required for centromere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA CENP-A forms an octa-meric complex with histones H4, H2A, H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.
References
1. Rieder C.L., et al. (1998) Trends Cell Biol. 8: 310-318.
2. Choo K.H. (2000) Trends Cell Biol. 10: 182-188.
3. Muro Y., et al. (2000) Clin. Exp. Immunol. 120: 218-223.
4. Howman E.V., et al. (2000) Proc. Natl. Acad. Sci. USA 97: 1148-1153.
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