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GRP170 Antibody
GRP170 Antibody, Clone 6E3-2C3
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC/IF |
|---|---|
| Primary Accession | Q9Y4L1 |
| Other Accession | NP_006389.3 |
| Host | Mouse |
| Isotype | IgG2b |
| Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Description | Mouse Anti-Human GRP170 Monoclonal IgG2bK |
| Target/Specificity | Detects ~170kDa. |
| Other Names | ORP150 Antibody, HSP12A Antibody, Hypoxia up regulated 1 Antibody, Orp150 Antibody, Glucose regulated Antibody 170 Antibody, 150kDa oxygen regulated Antibody Antibody |
| Clone Names | 6E3-2C3 |
| Immunogen | Raised against a synthetic peptide of human GRP170 |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.1% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 1 µg/ml of SMC-232 was sufficient for detection of GRP170 in 20 µg of HEK293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Endoplasmic Reticulum | Endoplasmic Reticulum Lumen |
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Provided below are standard protocols that you may find useful for product applications.
Background
GRP170, also known as ORP150, is the largest member of glucose-regulated Antibodys, and acts as a human chaperone Antibody. It is thought to play an important role in Antibody folding and secretion in the ER. Suppression of the Antibody is associated with accelerated apoptosis, therefore having an important cryoprotective role in hypoxia-induced cellular pertubation. This cryopotective role has led to an anti-tumor immune response, which will hopefully lead to therapeutic immunizations against cancers (1). GRP170 has also been shown to bind with dendritic cells and provide the danger signals to induce anti-tumor immune responses (2).
References
1. Wang H., et al. (2014) Front Oncol. 4: 377.
2. Manjili M.H., et al. (2006) Immun. Cell Biol. 84: 203-208.
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