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Notch1 Antibody
Notch1 Antibody, Clone S253-32
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC/IF |
|---|---|
| Primary Accession | Q01705 |
| Other Accession | NP_032740.3 |
| Host | Mouse |
| Isotype | IgG1 |
| Reactivity | Mouse, Rat |
| Clonality | Monoclonal |
| Description | Mouse Anti-Mouse Notch1 Monoclonal IgG1 |
| Target/Specificity | Detects >270kDa, ~120kDa and small fragments due to proteolysis. Does not cross-react with Notch 2 or Notch3. |
| Other Names | Neurogenic locus notch homolog protein 1 Antibody, hN1 Antibody, Translocation associated notch protein Antibody, TAN1 Antibody, TAN-1 Antibody, Motch A Antibody, mT14 Antibody, p300 Antibody, Motch Antibody, Mis6 Antibody, Neurogenic locus notch protein homolog Antibody, NICD Antibody, Notch 1 intracellular domain Antibody, Notch gene homolog 1 (Drosophila) Antibody, Notch homolog 1 Antibody, translocation-associated (Drosophila) Antibody, NOTCH Drosophila Antibody, homolog of 1 Antibody, Translocation associated notch homolog Antibody, Translocation associated notch protein TAN 1 Antibody, xotch Antibody |
| Clone Names | S253-32 |
| Immunogen | Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1 |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 1 µg/ml of SMC-430 was sufficient for detection of Notch1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Cell Membrane | Nucleus |
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Provided below are standard protocols that you may find useful for product applications.
Background
Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
References
1. Swiatek P.J., et al. (1994) Genes Dev. 8: 707-719.
2. Simpson P. (1994) The Notch Receptors. Austin, TX: R.G. Landes Company.
3. Lindsell C.E., et al. (1995) Cell 80: 909-917.
4. Uyttendaele H., et al. (1996) Development 122: 2251-2259.
5. Girard L., et al. (1996) Genes Dev. 10: 1930-1944.
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