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GluA1/GluR1 Glutamate Receptor Antibody

GluA1/GluR1 Glutamate Receptor Antibody, Clone S355-1

     
  •  - GluA1/GluR1 Glutamate Receptor Antibody ASM10274
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluA1/GluR1 Monoclonal Antibody, Clone N355/1 (ASM10274). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluA1/GluR1 Monoclonal Antibody (ASM10274) at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluA1/GluR1 Antibody (D) Composite.
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  • WB - GluA1/GluR1 Glutamate Receptor Antibody ASM10274
    Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa GluA1-GluR1 protein using Mouse Anti-GluA1-GluR1 Monoclonal Antibody, Clone N355/1 (ASM10274). Load: 10 µg. Block: 5% milk + TBST. Primary Antibody: Mouse Anti-GluA1-GluR1 Monoclonal Antibody (ASM10274) at 1:2000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC
Primary Accession P19490
Other Accession NP_113796
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Description Mouse Anti-Rat GluA1/GluR1 Glutamate Receptor Monoclonal IgG1
Target/Specificity Detects ~100kDa. Does not cross-react with GluR2.
Other Names AMPA 1 Antibody, AMPA selective glutamate receptor 1 Antibody, Glutamate receptor 1 Antibody, GluA1 Antibody, GLUH 1 Antibody, GluR K1 Antibody, GluRK1 Antibody, Glr-1 Antibody, Glur-1 Antibody, HIPA Antibody, Glur1 Antibody, GluR-A Antibody, AI853806 Antibody, 2900051M01Rik Antibody, GluRA Antibody, Glr1 Antibody, MGC13325 Antibody, GLUH1 Antibody, GluA1 Antibody, GLUR1 Antibody, HBGR1 Antibody, GLURA Antibody, gluR- Antibody, GluA1 Antibody, Glutamate receptor ionotropic AMPA 1 Antibody, Glutamate receptor ionotropic Antibody, Gria 1 Antibody, HBGR1 Antibody, OTTHUMP00000160643 Antibody, OTTHUMP00000165781 Antibody, OTTHUMP00000224241 Antibody, OTTHUMP00000224242 Antibody, OTTHUMP00000224243 Antibody
Clone Names S355-1
Immunogen Fusion protein amino acids 1-389 (extracellular N-terminus) of rat GluA1/GluR1
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.1% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SMC-440 was sufficient for detection of GluA1/GluR1 in 20 µg of mouse brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cell Membrane | Endoplasmic Reticulum | Cell Junction
Research Areas
Citations (0)
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Background

Glutamic acid is the major excitatory neurotransmitter in the mammalian central nervous system. Glutamate receptors are classified on the basis of their activation by different agonists (1-3). GluR1, human glutamate receptor type 1, is an integral membrane protein that is widely expressed in the human brain. The postsynaptic actions of glutamic acid are mediated by a variety of receptors that are named according to their selective agonists. GluR1 is known to bind a kainate subtype of agonist. It has been found that malfunctioning of the glutamatergic system may result in certain brain disorders and neurodegeneration (3).

References

1. Potier M.C., Spillantini M.G., Carter N.P. (1992) DNA Seq. 2(4): 211-218.
2. Puckett, C., et al. (1991) Proc. Nat. Acad. Sci. USA 88(17): 7557-7561.
3. Gregor, P., et al. (1993) Proc. Nat. Acad. Sci. USA 90: 3053-3057.

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Cat# ASM10274
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