GABA A Receptor Alpha 5 Antibody
GABA A Receptor Alpha 5 Antibody, Clone S415-24
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, ICC |
---|---|
Primary Accession | P31644 |
Other Accession | NP_000801.1 |
Host | Mouse |
Isotype | IgG1 |
Reactivity | Human, Mouse, Rat |
Clonality | Monoclonal |
Description | Mouse Anti-Human GABA A Receptor Alpha5 Monoclonal IgG1 |
Target/Specificity | Detects ~55kDa. Does not cross-react with GABA A Receptor Alpha2 or other GABA-A Receptors based on KO validation data. |
Other Names | Gamma-aminobutyric acid receptor subunit alpha-5 Antibody, GABA(A) Receptor subunit Alpha-5 Antibody, Gabra5 Antibody |
Clone Names | S415-24 |
Immunogen | Fusion protein amino acids 368-419 (last cytoplasmic domain before extracellular C-terminus) of human GABA A Receptor Alpha5. |
Purification | Protein G Purified |
Storage | -20ºC |
Storage Buffer | PBS pH7.4, 50% glycerol, 0.1% sodium azide |
Shipping Temperature | Blue Ice or 4ºC |
Certificate of Analysis | A 1:100 dilution of SMC-494 was sufficient for detection of GABA-A R, Alpha5 in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Cellular Localization | Cell Junction | Synapse | Postsynaptic Cell Membrane | Cell Membrane |
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Provided below are standard protocols that you may find useful for product applications.
Background
The GABA A Receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (1). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (2). Recent research shows their usage in investigating side effects of drugs and the effects of learning and memory. Inverse agonists of the Alpha5 subunit may be useful in the treatment of Alzheimer's Disease (3).
References
1. Bracamontes J.R. and Steinbach J.H. (2008) J Bio Chem. 283: 26128-26136.
2. Macdonald R.L., Olsen R.W. (1993) Annu Rev Neurosci. 17: 569-602.
3. Hengen K.B., et al. (2012) PLoS One. 7(1): e30608.
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