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Akt2 (PKB beta) Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC |
|---|---|
| Primary Accession | P31751 |
| Other Accession | NP_001617.1 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat, Rabbit, Hamster, Monkey, Pig, Chicken, Bovine, Xenopus, Dog, Sheep, Guinea Pig |
| Clonality | Polyclonal |
| Description | Rabbit Anti-Human Akt2 (PKB beta) Polyclonal |
| Target/Specificity | Detects ~65kDa. Does not react with PKBα (Akt1) or PKBγ (Akt3). |
| Other Names | AKT2 Antibody, PKB Antibody, PKB beta Antibody, PRKBB Antibody, protein kinase Akt2 Antibody, RAC BETA Antibody |
| Immunogen | A five residue synthetic peptide based on the human Akt2, coupled to KLH |
| Purification | Protein A Purified |
| Storage | -20ºC |
| Storage Buffer | TBS, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | A 1:3000 dilution of SPC-148 was sufficient for detection of PKBβ in 20 µg of HeLa cell lysate by ECL immunoblot analysis. |
| Cellular Localization | Cytoplasm | Nucleus | Cell Membrane |
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Provided below are standard protocols that you may find useful for product applications.
Background
Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1-3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3).
References
1. Downward J. (1998). Curr Opin Cell Biol. 10(2): 262-7.
2. Brazil D.P., and Hemmings B.A. (2001). Biochem Sci. 26(11):657-64.
3. Nicholson K.M., Anderson N.G. (2002). Cell Signal. 14(5): 381-95.
4. Hardie, G., Hanks, S. (1995). The Protein Kinase Facts Book, Protein-Serine Kinases Academic Press Limited, San Diego, CA 418pps.
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