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HSP60 (P. falciparum) Antibody

     
  • WB - HSP60 (P. falciparum) Antibody ASM10446
    Western blot analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody (ASM10446). Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (ASM10446) at 1:1000.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC/IF
Primary Accession P34940
Other Accession XM_001347402.1
Host Rabbit
Reactivity P. falciparum, Bacteria
Clonality Polyclonal
Description Rabbit Anti-P. falciparum HSP60 (P. falciparum) Polyclonal
Target/Specificity Detects ~ 60kDa. Cross-reacts with E.coli HSP60, GroEl.
Other Names CH60_PLAFG Antibody, Chaperonin CPN60 Antibody, mitochondrial Antibody
Immunogen Recombinant full length PfHSP60
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 0.9 µg/ml of SPC-185 was sufficient for detection of PfHSP60 in 20 µg of P. falciparum lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Mitochondrion | Mitochondrion Matrix
Citations (0)

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Background

In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).

References

1. Hartl F.U. (1996) Nature 381: 571-579.
2. Bukau B., and Horwich A.L. (1998) Cell 92: 351-366.
3. Hartl F.U and Hayer-Hartl M. (2002) Science 295: 1852-1858.
4. Jindal S., et al. (1989) Molecular and Cellular Biology 9: 2279-2283.
5. La Verda D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71.
6. Itoh H., et al. (2002) Eur. J. Biochem. 269: 5931-5938.
7. Gupta S. and Knowlton A.A. J. Cell Mol Med. 9: 51-58.
8. Deocaris C.C., et al. (2006) Cell Stress Chaperones 11: 116-128.
9. Lai H.C., et al. (2007) Am. J. Physiol. Endocrinol. Metab 292: E292-E297.

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Cat# ASM10446
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