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BVR Antibody

  • ICC/IF - BVR Antibody ASM10474
    Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BVR Polyclonal Antibody (ASM10474). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-BVR Polyclonal Antibody (ASM10474) at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Exosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-BVR Antibody. (C) Composite.
  • WB - BVR Antibody ASM10474
    Western blot analysis of Human, Rat Brain cell lysates showing detection of BVR protein using Rabbit Anti-BVR Polyclonal Antibody (ASM10474). Lane 1: Rat Brain. Lane 2: Human Brain lysates. Load: 10 µg. Primary Antibody: Rabbit Anti-BVR Polyclonal Antibody (ASM10474) at 1:1000.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P53004
Other Accession NP_000703.2
Host Rabbit
Reactivity Human
Clonality Polyclonal
Description Rabbit Anti-Human BVR Polyclonal
Target/Specificity Detects ~40-42kDa.
Other Names Biliverdin Reductase Antibody, BIEA_HUMAN Antibody, Biliverdin IX alpha reductase Antibody, Biliverdin reductase A Antibody, Biliverdin-IX alpha-reductase Antibody, BLVR A Antibody, BLVR Antibody, Blvra Antibody, BVR A Antibody, BVRA Antibody, Zinc metalloprotein Antibody, zinc-metalloprotein Antibody
Immunogen Human native full-length BVR
Purification Protein A Purified
Storage -20ºC
Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 1 µg/ml of SPC-214 was sufficient for detection of BVR in 10 µg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm
Research Areas
Citations (0)

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Biliverdin Reductase (BVR) is a cytoplasmic enzyme that catalyzes the conversion of biliverdin to bilirubin by converting a double bond between the second and third pyrrole ring into a single bond (1). It is ubiqutiously expressed in all tissues- it occurs in cells and brain regiuons that already display HO-1 and HO-2, but also in regions and cell types with potential to induce stress proteins. It is unique among all enzymes in having two pH optima, using a different cofactor at each pH range, NADH at pH7.0 and NADPH at pH8.7 (2). It is not inactivated by heat shock, and have shown to abate inflammation, oxidative stress and apoptosis (3).


1. Singleton J.W., Laster L. (1965). J Biol Chem. 240: 4780-4789.
2. Kutty R.K., Maines M.D. (1981) J Biol Chem. 256: 3956-3962.
3. Mishra M., Ndisand J.F. (2014) Curr Pharm Des. 20(9): 1370-1391.

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Cat# ASM10474
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