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ATG4D Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC |
|---|---|
| Primary Accession | Q86TL0 |
| Other Accession | NP_001268433.1 |
| Host | Rabbit |
| Reactivity | Human |
| Clonality | Polyclonal |
| Description | Rabbit Anti-Human ATG4D Polyclonal |
| Target/Specificity | Detects ~52kDa. |
| Other Names | APG4 autophagy 4 homolog D Antibody, APG4 D Antibody, ATG4 autophagy related 4 homolog D Antibody, AUTL4 Antibody, Cysteine protease ATG4D Antibody, MGC31226 Antibody |
| Immunogen | Synthetic peptide from the C-terminal of human ATG4D |
| Purification | Peptide Affinity Purified |
| Storage | -20ºC |
| Storage Buffer | PBS, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | A 1:1000 dilution of SPC-634 was sufficient for detection of ATG4D on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody. |
| Cellular Localization | Cytoplasm |
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Provided below are standard protocols that you may find useful for product applications.
Background
ATG4D, Auotphagy related 4D, cysteine peptidases, belong to the autophagy-related protein 4 family of C54 endopeptidases. Members of this family play a role in the biogenesis of autophagosomes (1). Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (2).
References
1. "Entrez Gene: Autophagy related 4D, cysteine peptidase".
2. Li M., et al. (2011) J Biol Chem. 286: 7327-7338.
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