|Application ||WB, ICC/IF|
|Description||Rabbit Anti-Human ATG4D Polyclonal|
|Other Names||APG4 autophagy 4 homolog D Antibody, APG4 D Antibody, ATG4 autophagy related 4 homolog D Antibody, AUTL4 Antibody, Cysteine protease ATG4D Antibody, MGC31226 Antibody|
|Immunogen||Synthetic peptide from the C-terminal of human ATG4D|
|Purification||Peptide Affinity Purified|
|Storage Buffer||PBS, 50% glycerol, 0.09% sodium azide|
|Shipping Temperature||Blue Ice or 4ºC|
|Certificate of Analysis||A 1:1000 dilution of SPC-634 was sufficient for detection of ATG4D on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.|
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Provided below are standard protocols that you may find useful for product applications.
ATG4D, Auotphagy related 4D, cysteine peptidases, belong to the autophagy-related protein 4 family of C54 endopeptidases. Members of this family play a role in the biogenesis of autophagosomes (1). Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (2).
1. "Entrez Gene: Autophagy related 4D, cysteine peptidase".
2. Li M., et al. (2011) J Biol Chem. 286: 7327-7338.
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