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c-Myc Antibody

Rabbit Anti-Human c-Myc Polyclonal

     
  •  - c-Myc Antibody ASM10575
    Immunohistochemistry analysis using Rabbit Anti-c-Myc Polyclonal Antibody (SPC-726). Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-c-Myc Polyclonal Antibody (SPC-726) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48.8 kDa c-Myc protein using Rabbit Anti-c-Myc Polyclonal Antibody (SPC-726). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Myc Polyclonal Antibody (SPC-726) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.8 kDa. Other Band(s): due to post translational modifications.
    detail
  •  - c-Myc Antibody ASM10575
    Immunohistochemistry analysis using Rabbit Anti-c-Myc Polyclonal Antibody (SPC-726). Tissue: Breast Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-c-Myc Polyclonal Antibody (SPC-726) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48.8 kDa c-Myc protein using Rabbit Anti-c-Myc Polyclonal Antibody (SPC-726). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-c-Myc Polyclonal Antibody (SPC-726) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.8 kDa. Other Band(s): due to post translational modifications.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC
Primary Accession P01106
Other Accession NP_002458.2
Host Rabbit
Clonality Polyclonal
Format c-Myc
Target/Specificity c-Myc
Other Names Phosphatidylinositol 3 kinase catalytic beta polypeptide Antibody, PIK3C1 Antibody, Phosphatidylinositol-4 Antibody, bHLHe39 Antibody, PI3Kbeta Antibody, PtdIns-3-kinase subunit p110-beta Antibody, p110 BETA Antibody, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform Antibody, OTTHUMP00000216901 Antibody, PK3CB_HUMAN Antibody, PI3K-beta Antibody, PI3K beta Antibody, p110Beta Antibody, Class E basic helix-loop-helix protein 39 Antibody, Proto-oncogene c-Myc Antibody, PI3-kinase subunit beta Antibody, OTTHUMP00000216904 Antibody, PI3KCB Antibody, Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta Antibody, Pik3cb Antibody, MGC133043 Antibody, 5-bisphosphate 3-kinase catalytic subunit beta isoform Antibody, PI3 kinase p110 subunit beta Antibody, PI3K Antibody, Transcription factor p64 Antibody, DKFZp779K1237 Antibody, MYC Antibody, BHLHE39 Antibody, PtdIns-3-kinase subunit beta Antibody, Phosphoinositide 3 kinase catalytic beta polypeptide Antibody, Myc proto-oncogene protein Antibody, PtdIns 3 kinase p110 Antibody, 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta Antibody
Clone Names c-Myc
Immunogen Synthetic peptide of Human c-Myc (220-320 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-726 was sufficient for detection of c-Myc in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Nucleus | Nucleoplasm | Nucleus | Nucleolus
Research Areas
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$ 330.00
Cat# ASM10575
11 Formats Available
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