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Cyclin D2 antibody

Rabbit Anti-Human Cyclin D2 Polyclonal

     
  •  - Cyclin D2 antibody ASM10582
    Immunohistochemistry analysis using Rabbit Anti-Cyclin D2 Polyclonal Antibody (SPC-733). Tissue: Stomach. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Cyclin D2 Polyclonal Antibody (SPC-733) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~33.1 kDa Cyclin D2 protein using Rabbit Anti-Cyclin D2 Polyclonal Antibody (SPC-733). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclin D2 Polyclonal Antibody (SPC-733) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~33.1 kDa. Other Band(s): ~50 kDa due to post translational modifications.
    detail
  •  - Cyclin D2 antibody ASM10582
    Immunohistochemistry analysis using Rabbit Anti-Cyclin D2 Polyclonal Antibody (SPC-733). Tissue: Stomach. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-Cyclin D2 Polyclonal Antibody (SPC-733) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection. | Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~33.1 kDa Cyclin D2 protein using Rabbit Anti-Cyclin D2 Polyclonal Antibody (SPC-733). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclin D2 Polyclonal Antibody (SPC-733) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~33.1 kDa. Other Band(s): ~50 kDa due to post translational modifications.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC
Primary Accession P30279
Other Accession NP_001750.1
Host Rabbit
Clonality Polyclonal
Format Cyclin D2
Target/Specificity Cyclin D2
Other Names CCND2 Antibody, ccnd2 Antibody, CCND2_HUMAN Antibody, G1/S-specific cyclin-D2 Antibody, CCND 2 Antibody, MGC102758 Antibody, CyclinD2 Antibody, G1/S specific cyclin D2 Antibody
Clone Names Cyclin D2
Immunogen Synthetic peptide from the C-terminal of Human Cyclin D2 (189-289 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-733 was sufficient for detection of Cyclin D2 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Nucleus | Cytoplasm | Membrane
Research Areas
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$ 330.00
Cat# ASM10582
11 Formats Available
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