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mTOR Antibody

Rabbit Anti-Human MTOR Polyclonal

     
  •  - mTOR Antibody ASM10647
    Western blot analysis of Human HeLa showing detection of 288.9 kDa MTOR protein using Rabbit Anti-MTOR Polyclonal Antibody (SPC-798). Load: 10 µg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-MTOR Polyclonal Antibody (SPC-798) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: 288.9 kDa. Other Band(s): 25 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P42345
Other Accession NP_004949.1
Host Rabbit
Clonality Polyclonal
Format MTOR
Target/Specificity MTOR
Other Names Rapamycin target protein 1 Antibody, FKBP rapamycin associated protein Antibody, Serine/threonine-protein kinase mTOR Antibody, Rapamycin associated protein FRAP2 Antibody, FLJ44809 Antibody, Mechanistic target of rapamycin Antibody, FRAP1 Antibody, RAFT1 Antibody, FK506 binding protein 12 rapamycin associated protein 1 Antibody, MTOR_HUMAN Antibody, dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) Antibody, FKBP12-rapamycin complex-associated protein Antibody, OTTHUMP00000001983 Antibody, EC:2.7.11.1 Antibody, FKBP12 rapamycin complex associated protein Antibody, FKBP12-rapamycin complex-associated protein 1 Antibody, Rapamycin and FKBP12 target 1 Antibody, MTOR Antibody, FK506 binding protein 12 rapamycin associated protein 2 Antibody, FK506-binding protein 12-rapamycin complex-associated protein 1 Antibody, RAPT1 Antibody, FK506 binding protein 12 rapamycin complex associated protein 1 Antibody, mTOR Antibody, FRAP2 Antibody, Mammalian target of rapamycin Antibody, FRAP Antibody, Rapamycin target protein Antibody
Clone Names MTOR
Immunogen Synthetic peptide from the mid-protein of Human Serine/threonine-protein kinase mTOR (aa. 740-840)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-798 was sufficient for detection of MTOR in 10 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Endoplasmic Reticulum Membrane | Peripheral Membrane Protein | Cytoplasmic Side | Golgi Apparatus Membrane | Peripheral Membrane Protein | Cytoplasmic Side | Mitochondrion Outer Membrane | Peripheral Membrane Protein | Cytoplasmic Side | Lysosome | Cytoplasm | Nucleus | Pml Body | Microsome Membrane
Research Areas
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$ 330.00
Cat# ASM10647
11 Formats Available
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