Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
GRP94 Antibody
GRP94 Antibody, Clone 6B12
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P14625 |
|---|---|
| Other Accession | NP_003290.1 |
| Host | Mouse |
| Clonality | Monoclonal |
| Target/Specificity | GRP94 |
| Other Names | HSP90B1 Antibody, GP96 Antibody, TRA1 Antibody, ECGP Antibody, 94 kDa glucose regulated protein antibody, 94 kDa glucose-regulated protein antibody, ECGP antibody, Endoplasmin antibody, Endothelial cell (HBMEC) glycoprotein antibody, ENPL_HUMAN antibody, Glucose regulated protein 94kDa antibody, gp96 antibody, gp96 homolog antibody, GRP 94 antibody, GRP-94 antibody, Heat shock protein 90 kDa beta member 1 antibody, heat shock protein 90kDa beta (Grp94), member 1 antibody, Heat shock protein, 90 kDa, beta, 1 antibody, HSP90B1 antibody, Stress inducible tumor rejection antigen GP96 antibody, TRA1 antibody, tumor rejection antigen (gp96) 1 antibody, Tumor rejection antigen 1 antibody, Tumor rejection antigen gp96 antibody, Tumor rejection antigen-1 (gp96) antibody |
| Clone Names | GRP94 |
| Immunogen | KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA) |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | A 1:1000 dilution of SMC-541 was sufficient for detection of GRP94 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Endoplasmic reticulum lumen | Melanosome |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).
References
1. Rudolph R.W., and Bedows E. (1997) J Biol Chem 272: 3125-3128.
2. Srivastava P.K., et al. (1994) Immunogenetics. 39(2):93-98.
3. Mazzarella R.A., and Green M. (1987) J Biol Chem 262: 8875-8883.
4. Kang, H.S. and Welch W.J. (1991) J Biol Chem 266(9): 5643-5649.
5. Soldano K.L., et al. (2003) J Biol Chem 278(48): 48330-48338.
6. Chu F., et al. (2006) Protein Sci 15(6): 1260-1269.
7. Peter F., et al., (1992) J Biol Chem 267: 10631-10637.
8. Allen S. et al. (2000) Blood 96(2): 560-568.
9. Sato K et al. (2001) Blood 98(6): 1852-1857.
10. Yun S.-W. et al (2000) Brain Research Bulletin.52(5): 371-378.
11. Choukhi A., et al. (1998) J. Virol. 72: 3851-3858.
12. Hoshino T., et al. (1998) Blood 91(11): 4379-4386.
13. Riera M. et al. (1999) Mol. Cell Biochem. 191: 97-104.
14. Gusarova V., et al. (2001) J. Biol. Chem. 276(27): 24891-24900.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
