Anti-MOUSE IgG2a ( Rhodamine Conjugated) Pre-adsorbed Secondary Antibody
Goat Polyclonal, Rhodamine (TRITC)
|Anti-MOUSE IgG2a (Gamma 2a chain) (GOAT) Antibody Rhodamine Conjugated (Min Cross Bv, Hu, and Rb Serum Proteins)
|3.1 moles Rhodamine (TRITC) per mole of IgG
|WB, IF, IC
IF Microscopy 1:1,000-1:5,000
Western Blot 1:2,000-1:10,000
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
|Mouse IgG2a heavy chain
|Restore with deionized water (or equivalent)
|10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
|0.01% (w/v) Sodium Azide
|MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
|Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
|This product is for research use only and is not intended for therapeutic or diagnostic applications.
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Mouse IgG2a secondary antibody is available in a variety of formats. Rhodamine secondaaries are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.