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Anti-Mouse IgG F(ab')2 (Peroxidase Conjugated) Pre-Adsorbed Secondary Antibody

Rabbit Polyclonal, Peroxidase (Horseradish)

     
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Product Information
Description Anti-MOUSE IgG F(ab')2 (RABBIT) Antibody Peroxidase Conjugated (Min X Human Serum Proteins)
Host Rabbit
Conjugate Peroxidase (Horseradish)
Target Species Mouse
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E, IC
Application Note ELISA 1:16,000
Western Blot 1:500-1:2,000
Immunochemistry 1:500-1:2,000
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG F(ab')2
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Mouse IgG F(ab')2 fragment
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum.  No reaction was observed against Mouse IgG F(c) or Human Serum Proteins.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
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$ 195.00
Cat# ASR2846
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Availability: 3-5 days
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