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Anti-Mouse IgM (H&L) Secondary Antibody
Rabbit Polyclonal, Unconjugated
- SPECIFICATION
- CITATIONS
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- BACKGROUND
| Description | Anti-MOUSE IgM (H&L) (RABBIT) Antibody |
|---|---|
| Host | Rabbit |
| Conjugate | Unconjugated |
| Target Species | Mouse |
| Clonality | Polyclonal |
Application 
| WB, E, IC |
| Application Note | ELISA 1:20,000-1:100,000 Western Blot 1:2,000-1:10,000 Immunochemistry 1:1,000-1:5,000 |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgM (H&L) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Mouse IgM whole molecule |
| Species of Origin | Mouse |
| Reconstitution Volume | 5.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | None |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Ambient |
|---|---|
| Purity | Anti-MOUSE IgM (H&L) (RABBIT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and Mouse IgM. No reaction was observed against Mouse IgG F(c). Some light chain cross reactivity may occur against Mouse IgG. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Mouse IgM antibody recognizes the mu chain of the Mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Mouse IgM and Mouse Serum. No reaction was observed against Mouse IgA or Mouse IgG. ELISA was used to confirm minimal cross reactivity against other mouse heavy or light chain isotypes. Anti-Mouse IgM antibody is ideal with investigators involved in serum component protein research.
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