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Anti-Guinea Pig IgG (H&L) (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody
Goat Polyclonal, Alkaline Phosphatase (Calf Intestine)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Description | Anti-GUINEA PIG IgG (H&L) (GOAT) Antibody Alkaline Phosphatase Conjugated (Min X Bv Ch Gt Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) |
|---|---|
| Host | Goat |
| Conjugate | Alkaline Phosphatase (Calf Intestine) |
| Target Species | Guinea Pig |
| Reactivity | Guinea Pig |
| Clonality | Polyclonal |
Application 
| WB, E, IC |
| Application Note | ELISA 1:2,000-1:10,000 Western Blot 1:500-1:2,500 Immunochemistry 1:200-1:1,000 |
| Physical State | Liquid (sterile filtered) |
| Host Isotype | IgG |
| Target Isotype | IgG (H&L) |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Immunogen | Anti-Guinea Pig IgG (H&L) was produced by repeated immunization with Guinea Pig IgG whole molecule in goat. |
| Shipping Condition | Wet Ice |
|---|---|
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. |
| Storage Condition | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Anti-Guinea Pig IgG (H&L) antibody generated in goat detects specifically guinea pig IgG (H&L). This secondary antibody anti-Guinea Pig is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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