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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   F(ab')2 Anti-MOUSE IgG (H&L) (Phycoerythrin Conjugated) Pre-adsorbed Secondary Antibody   

F(ab')2 Anti-MOUSE IgG (H&L) (Phycoerythrin Conjugated) Pre-adsorbed Secondary Antibody

Goat Polyclonal, R-Phycoerythrin (RPE)

     
  • DB - F(ab')2  Anti-MOUSE IgG (H&L)  (Phycoerythrin Conjugated) Pre-adsorbed Secondary Antibody ASR3194
    Dot Blot of Phycoerythrin conjugated Goat F(ab’)2 Anti Mouse IgG (H&L) Antibody. Lane 1: Mouse IgG. Load: A three-fold serial dilution starting at 200ng. Primary Antibody: None. Secondary Antibody: F(ab’)2 Anti-Mouse IgG (H&L) (GOAT) Antibody Phycoerythrin conjugated secondary antibody was used at 1:1000 for 1 Hour at RT. Blocking: 5% Blotto for 1 Hour at 20°C.
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Product Information
Description F(ab')2 Anti-MOUSE IgG (H&L) (GOAT) Antibody Phycoerythrin conjugated Min X Bv Hm, Hs, Hu Rb, Rt, & Sh Serum Proteins
Host Goat
Conjugate R-Phycoerythrin (RPE)
Target Species Mouse
Reactivity Mouse
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, FC
Application Note IF Microscopy 1:100-1:250
FlowCytometry 1:100-1:250
FLOW1:100-1:250
Physical State Lyophilized
Host Isotype IgG F(ab')2
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen F(ab')2 anti-Mouse IgG (H&L) was produced by repeated immunization with Mouse IgG whole molecule in goat.
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity F(ab')2 Anti-MOUSE IgG (H&L) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   F(ab')2 Anti-MOUSE IgG (H&L) (GOAT) Antibody assays by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine Chicken Goat Guinea Pig Horse Hamster Human Rabbit Rat and Sheep Serum Proteins.
Storage Condition Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
Citations (0)
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Background

F(ab')2 anti-Mouse IgG (H&L) antibody generated in goat detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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$ 278.00
Cat# ASR3194
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