F(ab')2 Anti-RABBIT IgG [H&L] (Phycoerythrin Conjugated) Pre-adsorbed Secondary Antibody
Donkey Polyclonal, R-Phycoerythrin (RPE)
|Description||F(ab')2 Anti-RABBIT IgG [H&L] (DONKEY) Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins|
|Application ||IF, FC|
|Application Note||IF Microscopy 1:100-1:200|
|Host Isotype||IgG F(ab')2|
|Target Isotype||IgG (H&L)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Anti-Rabbit IgG was produced by repeated immunization with Rabbit IgG whole moleculein goat.|
|Reconstitution Volume||1.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Sodium Azide|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.|
|Storage Condition||Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
F(ab')2 RABBIT IgG [H&L] Antibody Phycoerythrin conjugated (Pre-Adsorbed) was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 RABBIT IgG [H&L] Antibody Phycoerythrin conjugated (Pre-Adsorbed) is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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