Anti-Goat IgG F(ab')2 (Fluorescein Conjugated) Secondary Antibody
Rabbit Polyclonal, Fluorescein (FITC)
|Description||Anti-GOAT IgG F(ab')2 (RABBIT) Antibody Fluorescein Conjugated|
|FP Value||3.2 moles Fluorescein (FITC) per mole of IgG|
|Application ||IF, FC|
|Application Note||FLISA 1:10,000-1:50,000|
IF Microscopy 1:1,000-1:5,000
|Target Isotype||IgG F(ab')2|
|Buffer||0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Goat IgG F(ab')2 fragment|
|Reconstitution Volume||2.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Sodium Azide|
|Purity||This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG, Goat IgG F(ab’)2 and Goat Serum. No reaction was observed against Goat IgG F(c).|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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