MOUSE IgG (BULK ORDER)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Description | MOUSE IgG whole molecule (BULK ORDER) |
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Conjugate | Unconjugated |
Physical State | Lyophilized |
Host Isotype | IgG |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Species of Origin | Mouse |
Reconstitution Volume | 2.5 mL |
Reconstitution Buffer | Restore with deionized water (or equivalent) |
Stabilizer | None |
Preservative | 0.01% (w/v) Sodium Azide |
Shipping Condition | Ambient |
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Purity | Mouse IgG was prepared from normal mouse serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse Serum. |
Storage Condition | Store purified Mouse IgG at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mouse IgG purified protein (Immunoglobulin G) are antibody molecules. Mouse IgG is composed of four peptide chains — two heavy chains and two light chains. Mouse IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. Mouse IgG typically constitutes 75% of serum immunoglobulins. Mouse IgG molecules are synthesized and secreted by plasma B cells. Ideal as a negative control for Flow Cytometry, Western blotting, immunoprecipitation and immunohistochemistry applications.
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