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Anti-GLUTAMINE SYNTHETASE (Microbial Brevibacterium Sp) (GOAT) Antibody

Glutamine Synthetase Antibody

     
  •  - Anti-GLUTAMINE SYNTHETASE (Microbial Brevibacterium Sp) (GOAT) Antibody ASR3627
    Rockland Goat anti Glutamine Synthetase antibody (200-101-238 lot 8182) was used to detect Glutamine Synthetase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.
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Product Information
Host Goat
Conjugate Unconjugated
Target Species Microbial
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E, IP, I, LCI
Application Note Anti-Glutamine Synthetase has been tested by western blot and is suitable to be assayed against 1.0 ug of Glutamine Synthetase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.
Physical State Lyophilized
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Glutamine Synthetase [native from brevibacterium species]
Reconstitution Volume 2.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Other Names 3345165
Purity This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Glutamine Synthetase [Microbial].  Cross reactivity against Glutamine Synthetase from other sources is unknown.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Background

Catalytic activity of Glutamine Synthetase is ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

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$ 1,292.00
Cat# ASR3627
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