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Anti-ALDOLASE [Rabbit Muscle] (GOAT) Antibody
Aldolase Antibody
![WB - Anti-ALDOLASE [Rabbit Muscle] (GOAT) Antibody ASR3632](/assets/uploads/products/202407/ASR3632-Anti-ALDOLASE-Antibody-Muscle-GOAT-Antibody-WB-4x3_1.jpg)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Goat |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Rabbit |
| Reactivity | Human, Rabbit |
| Clonality | Polyclonal |
Application 
| WB, E, IP, I, LCI |
| Application Note | Anti-Aldolase has been tested by immunoprecipitation and western blot and is suitable to be assayed against 1.0 µg of Aldolase [Rabbit Muscle] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) code #605-4302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product. Use approximately 5 ul of antibody to immunoprecipitate 50 ul of protein lysate. |
| Physical State | Lyophilized |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Aldolase [Rabbit Muscle] |
| Reconstitution Volume | 2.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 100009055 |
|---|---|
| Other Names | 100009055 |
| Purity | This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Aldolase [Rabbit Muscle]. This antibody will detect human Aldolase. Cross reactivity against Aldolase from other tissues and species may also occur. It has been reported that this antibody can detect human Aldolase on immunoblot showing a 41 kDa band in lysates from MCF7, NMB231 and HBL100 cell lines. |
| Storage Condition | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | ALDOA |
|---|---|
| Function | Catalyzes the reversible conversion of beta-D-fructose 1,6- bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis (PubMed:17329259, PubMed:20129922). In addition, may also function as scaffolding protein (PubMed:17329259). |
| Cellular Location | Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, M line. Note=In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+) |
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Provided below are standard protocols that you may find useful for product applications.
Background
Aldolase plays a key role in glycolysis and gluconeogenesis. In addition, it may also function as scaffolding protein. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in the liver, and aldolase C in the brain. Alkylation of Arg-43 inactivates the enzyme. Aldolase is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
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