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Anti-NF-Y (B subunit) (RABBIT) Antibody
NF-Y Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Human |
| Clonality | Polyclonal |
Application 
| WB, IHC, E, I, LCI |
| Application Note | This product was tested by immunoblot and found to be reactive against NF-Y (B subunit specific) at a dilution of 1:2,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-NF-Y (B subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y. Minimal reactivity was observed against the A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against NF-Y using 0.5 to 1.0 µl per assay. This product is tested for immunohistochemistry in human thymus tissue at 1:500. |
| Physical State | Liquid (sterile filtered) |
| Immunogen | NF-Y (B subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 4801 |
|---|---|
| Other Names | 4801 |
| Purity | This product was prepared from monospecific antiserum by delipidation and defibrination. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | NFYB |
|---|---|
| Synonyms | HAP3 |
| Function | Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. |
| Cellular Location | Nucleus. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Nuclear transcription factor Y subunit beta is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors.
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